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\textbf{Fig. 9} HeLa cells were infected with \textit{S. Typhi} Ty2W at ~40 MOI for various times. (Left) The plate count assay method followed the steps as described in Fig. 8, but with invasion time ranging form 0 to 60 minutes. (Right) Another set of time course-infected monolayers were examined using AO-CV staining (viable intracellular bacteria stain bright green, nonviable intracellular bacteria stain red/orange, and extracellular bacteria counterstain deep violet/black). The second column gives mean bacteria per infected cell, and in brackets the predicted number of bacteria for all cells after an hour of growth. This is included for comparison to the plate count assay bacteria/cell, which involves a 1 hour gentamicin kill period during which intracellular bacteria double according to control studies (data not shown). For example, the calculation for the 10 minute data would be (1.9 bacteria/cell) x (10\% of cells) x (2) \approx 0.4 bacteria/cell