David Coil edited Materials_and_Methods_Sample_collection__.md  over 8 years ago

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Once the plates arrived, we thawed all six plates and performed a high-density measurement in a Tecan M200 plate reader. OD600 readings were taken in a 5x5 grid covering the entire well, these 25 measurements were then averaged within each well.   ***Analysis***  For each sample, the averages of the six space replicates and six ground replicates were compared using a Student's t-test. To correct for multiple hypothesis testing, the p-values were adjusted using the False Discovery Rate (FDR) method \cite{1023072346101}.  ***Confirmation***