deletions | additions       diff --git a/Materials_and_Methods_Sample_collection__.md b/Materials_and_Methods_Sample_collection__.md  index baa12dc..6e5b3a3 100644  --- a/Materials_and_Methods_Sample_collection__.md  +++ b/Materials_and_Methods_Sample_collection__.md 
...
***Confirmation***  In order to confirm that the observed results were not due to contamination of the wells, each of the 12 replicates (6 space, 6 ground) for bacteria showing statistically different growth between the ISS and earth were cultured after the experiment. Bacteria were struck from the wells onto LB-agar plates, then single colonies were grown into overnight cultures. DNA was extracted (Wizard ???) using a Wizard Genomic DNA Purification kit (Promega)  from each of the 36 cultures and the identity confirmed with PCR and Sanger sequencing using the 27F and 1391 primers as described above.