deletions | additions       diff --git a/Materials_and_Methods_Sample_collection__.md b/Materials_and_Methods_Sample_collection__.md  index db50f91..8d11ed5 100644  --- a/Materials_and_Methods_Sample_collection__.md  +++ b/Materials_and_Methods_Sample_collection__.md 
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***Comparison to ISS swab data***  The bacterial community on the ISS was recently surveyed by PCR amplification and sequencing of 16S rRNA genes from swabs (Lang et al 2015). We compared the 16S sequence of each of our bacterial isolates to the "representative sequence" from each OTU operational taxonomic unit (OTU)  generated from the survey data. A local BLAST was performed, limiting the results to 97% identity over at least 250 bp of the rRNA sequence (the amplified fragment is 253 bp).