deletions | additions       diff --git a/Materials_and_Methods_Sample_collection__.md b/Materials_and_Methods_Sample_collection__.md  index b7855b8..1a263c4 100644  --- a/Materials_and_Methods_Sample_collection__.md  +++ b/Materials_and_Methods_Sample_collection__.md 
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This payload was flown on the CRS-3 launch of the Space X Dragon spacecraft, on a Falcon 9 v1.1 rocket which successfully launched April 18, 2014. After a few days the plates were removed from the MELFI (Minus Eighty Lab Freezer For ISS) and partially thawed. However, technical problems arose and the plates were placed back into the MELFI until December 8, 2014. At that time, all three plates were thawed and the OD600 of each well (3x3 grid) was measured at time 0 (60 minutes after removal from the freezer) and then every 24 hours for 4 days. Measurements were performed in a Molecular Devices SpectraMax M5e plate reader which was modified for integration onto the ISS. On these same days, equivalent measurements were taken in a Molecular Devices SpectraMax M5e plate reader at UC Davis. After the experiment the "ground" plates were placed back at -80 °C and the "space" plates were placed back into the MELFI. In February 2015, the space plates were transferred to a -95 °C freezer on board a Dragon spacecraft and splashdown in the Pacific Ocean occurred on Feb 10th 2015. The plates were then mailed to UC Davis on dry ice and were transferred to -80 °C when received.  Once the plates were returned from the ISS, returned,  we thawed out all 6 plates and performed a high-density measurement in a Tecan M200 plate reader. OD600 readings were taken in a 5x5 grid covering the entire well, these 25 measurements were then averaged within each well. ***Analysis***  For each sample, the averages of the 6 space replicates and 6 ground replicates were compared using a Student's t-test. To correct for multiple hypothesis testing, the p-values were adjusted using the False Discovery Rate (FDR) method.