David Coil edited Methods.md  over 9 years ago

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CATALASE AND OXIDASE?  Carbon source utilization was assayed using the Phenotypic MicroArray(TM) services offered by Biolog Inc. METHODS STUFF HERE. Plate PM1 Inc following standard proceedures for gram-negative bacteria. Colonies were grown on blood agar at room temperature and suspended in IF-0a inoculating fluid (Biolog) to a density of 42% transmittance. The cell suspension  was performed diluted 1:6  in triplicate, IF-0a plus 1x Dye H (Biolog)  and a  carbon source utilization MicroPlate (PM1; Biolog) was inoculated with 100μl per well. The PM1 microplate  was only scored if growth incubated at 23 degrees C and read by the OmniLog instrument every 15 minutes for 96 hours. Duplicate sets of OmniLog data were converted to average read value and a threshold of 78  was observed required  in all three plates. both replicates for a positive call.  **Respiratory quinones, polar lips, and fatty acids**