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David Coil edited Methods.md
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Cells were initially grown on plates containing either Reasoner´s 2A agar (R2A), or lysogeny broth agar (LB). A clear preference for LB was observed and so was used for all subsequent experiments. Salt tolerance was measured in liquid media (25C) at 0%, 1%, 3%, 5%, 8%, 10%, 12%, 15%, and 20% w/v NaCl. pH tolerance was measured in liquid media (25C) at pH 3.4, 4.0, 4.9, 5.5, 6.3, 7.9, 8.2, 8.8, and 9.1. Temperature preference was measured in liquid culture across the range 4C to 30C.
Cell
morphology morphology, motility, and presence/absence of flagella was examined by light microscopy (Zeiss Axio Lab.A1) and transmission electron
microscopy. microscopy (TEM). Cell cultures in either exponential or stationary phase were
negatively stained (GET METHODS HERE, THERE WERE TWO KINDS, Moly prepared for TEM by the UC Davis Electron Microscopy lab as follows. 400 mesh copper grids with formvar/carbon support film (Ted Pella, Inc., Redding, Ca.) were placed on dental wax. A 10μl drop of fixed of unfixed sample was placed onto the grid and left in a dust-free environment for 10 min. Then excess was wicked off with filter paper. A 10μl drop of 1% PTA pH 5.8 (phosphotungstic acid) or 1% ammonium molybdate in DDH2O was added to the grid and
PTA). wicked off immediately. Grids were allowed to air-dry completely before viewing in a Philips CM120 (FEI/Philips Inc, Hillsborough, Or.) electron microscope at 80KV.
Catalase and oxidase activities were observed (SAME AS CLASSIC?)