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David Coil edited Methods.md
over 9 years ago
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**16S rDNA and Genome Sequencing**
Genomic DNA was extracted using a Wizard Genomic DNA Purification Kit (Promega). A nearly full-length 16S sequence was amplified using the 27F (5'-AGAGTTTGATCMTGGCTCAG-3') and 1391R (5'-GACGGGCGGTGTGTRCA-3') "universal" primers. Sanger sequencing was provided by the College of Biological Science UC-DNA Sequencing Facility (UC Davis). This DNA was also used for Illumina sequencing of the draft genome as described elsewhere (Coil and Eisen, STATUS?). The genome sequence was annotated using the RAST
server, this annotation was used to determine the presence of (WHAT ARE WE DOING HERE?). server \cite{Aziz_2008} \cite{Overbeek_2013}.
A phylogentic tree was constructed using all type strains from the
Erythrobacteraceae _Erythrobacteraceae_ family. The 1491bp 16S rDNA sequence was obtained from the genome assembly in RAST, and uploaded to the Ribosomal Database
Project\cite{Cole_2013}. Project \cite{Cole_2013}. An alignment