David Coil edited Results and Discussion.md  over 9 years ago

Commit id: 4bd375fb845ed2242bc225ecfd7620afda1faa8b

deletions | additions      

       

**Phylogeny and Genome analysis**  Phylogenetic analysis was performed using the full length (1491bp) 16S rDNA sequence from the genome assembly, not the shorter (1350bp) version from Sanger sequencing. The Coronado(T) 16S rDNA sequence showed only 95% identity to the phylogenetically closest relative, _Porphyrobacter sanquineus_, and identity was even lower throughout the rest of the tree. Given the low 16S rDNA identity to other members of the family, we did not perform DNA-DNA hybridization as this would have been uninformative (\cite{STACKEBRANDT_1994}, \cite{STACKEBRANDT_1994},  \cite{Tindall_2010} Our phylogentic tree of type strains (FIGURE ?) from the _Erythrobacteraceae_ family shows that the Coronado(T) strain falls within a clade containing all members of the _Porphyrobacter_ genus, the sole representative of _Erythromicrobium_, and one _Erythrobacter_ (_Erythrobacter litoralis_) which is probably mis-classified given its position in the tree. As discussed above, a large-scale taxonomic revision of the family is most likely in order given the totally screwed up tree (JENNA, MORE DETAILS HERE). Based on this tree, we chose to compare Coronado(T) to these three genera in Table 1.