David Coil edited Results and Discussion.md  over 9 years ago

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Cells were not observed to form spores or possess flagella, though a number of genes for flagellar biosynthesis are present in the genome.  Temp CELL MEASUREMENTS HERE  Growth was only observed under aerobic conditions, from 4C to 28C, with optimal growth around 25C. Low levels of growth were observed at  pH microscopy 5.5 and 7.9, maximum growth occured around neutral pH. Salt was not required for growth, and the strain could tolerate up to 5% NaCl, with optimal growth at 1% NaCl.  **Phylogeny and Genome analysis**  Phylogenetic analysis was performed using the full length (1491bp) 16S rDNA sequence from the genome assembly, not the shorter (1350bp) version from Sanger sequencing. The Coronado(T) 16S rDNA sequence showed only 95% identity to the phylogenetically closest relative, _Porphyrobacter sanquineus_, and identity  was even lower throughout the rest of the tree. Given the low 16S rDNA identity to other members of the family, we did not perform DNA-DNA hybridization as this would have been uninformative (\cite{STACKEBRANDT_1994}, \cite{Tindall_2010} Our phylogentic tree of type strains (FIGURE ?) from the _Erythrobacteraceae_ family shows that the Coronado(T) strain falls within a clade containing all members of the _Porphyrobacter_ genus, the sole representative of _Erythromicrobium_, and one _Erythrobacter_ (_Erythrobacter litoralis_) which is probably mis-classified given its position in the tree. As discussed above, a large-scale taxonomic revision of the family is most likely in order given the totally screwed up tree (JENNA, MORE DETAILS HERE). Based on this tree, we chose to compare Coronado(T) to these three genera in Table 1.  16S tree stuff here, results from Genome analysis  **Polar lipid, respitory lipoquinone, and fatty acid methyl esters**  The major cellular fatty acids of strain Coronado(T) are C18:1w7cis (56.6%) and C16:0 (20.3%). Other fatty acids found in significant amounts (>2%) are 2-OH-C14:0 (4.8%), C16:1w7cis (9.8%), and C17:1w6cis (2%). The fatty acid profile of strain Coronado(T) fits generally within the ranges described for members of the most closely related genera (_Erythrobacter_, _Porphyrobacter_ and _Erythromicrobium_, comparison data from \cite{Hiraishi_2002}). Two exceptions to this are a slighly higher level of C14:0 than average and a much higher level of C16:0 than average.  The major respiratory quinone is Q10 (92%), as for all members of the _Erythrobacteraceae_ family. The predominant polar lipid is phosphatidylglycerol, with significant amounts of sphingoglycolipid and phosphatidylethanolamine. Smaller amounts of diphosphatidylglycerol, phosphatidylcholine, and two unidentified phospholipids were also observed.