David Coil edited Results and Discussion.md  over 9 years ago

Commit id: d8d8373674237cfd23889f88b105be804a67afe2

deletions | additions      

       

**Morphological, physiological, and biochemical characteristics**  Cells were non-motile and not observed to form spores or possess flagella, though over 30 flagella or flagella-associated genes are present in the genome. In contrast, the genome for a non-motile close relative (_P. cryptus_) does not contain any flagellar genes so it is possible that strain Coronado(T) is motile under specific conditions. Strain Unlike most members of the _Erythrobacteraceae family_, strain  Coronado(T) is oxidase/catalase negative oxidase  and catalase negative. This strain is also  unable to hydrolze casein or starch.IMPLICATIONS OF CATALASE.  Cells were oval or rod shaped and ranged in length from 1.2um to 2.2um with an average of 1.6um (Figure XX). Cell width ranged from .6um to 1um with an average of .8um.  Growth was only observed under aerobic conditions, from 4C to 28C, with optimal growth around 25C. Low levels of growth were observed at pH up to pH 8.0, maximum growth occured around neutral pH. Salt was required for growth, and the strain could not grow at 3% NaCl, optimal growth was at 1% NaCl.  Strain Coronado(T) could utilize the following as sole carbon sources: Glycyl-L-Glutamic Acid, L-Rhamnose, D-Mannose, D-Trehalose, a-D-Glucose, L-Fucose, D-Galactose, Citric acid, D-Glucuronic acid, D-Galactonic acid, L-Galactonic acid-g-Lactone, acid-\(\gamma\)-Lactone,  Acetoacetic acid, Acetic acid, Pyruvic acid, and L-Malic acid. The strain was unable to grow on N-Acetyl-D-Glucosamine, D-Saccharic Acid, Succinic Acid, L-Aspartic Acid, L-Proline, D-Alanine, Dulcitol, D-Serine, D-Sorbitol, Glycerol, D-Gluconic Acid, D,L-\(\alpha\)-Glycerol-Phosphate, L-Lactic Acid, Formic Acid, D-Mannitol, L-Glutamic Acid, D-Glucose-6-Phosphate, D-Galactonic Acid-\(\gamma\)-Lactone, D,L-Malic Acid, Tween 20, D-Fructose, Maltose, D-Melibiose, Thymidine, L-Asparagine, D-Aspartic Acid, D-Glucosaminic Acid, 1,2-Propanediol, Tween 40, \(\alpha\)-Keto-Glutaric Acid, \(\alpha\)-Keto-Butyric Acid, \(\alpha\)-Methyl-D-Galactoside, \(\alpha\)-D-Lactose, Lactulose, Sucrose, Uridine, L-Glutamine, m-Tartaric Acid, D-Glucose-1-Phosphate, D-Fructose-6-Phosphate, Tween 80, \(\alpha\)-Hydroxy Glutaric Acid-\(\gamma\)-Lactone, \(\alpha\)-Hydroxy Butyric Acid, \(\beta\)-Methyl-D-Glucoside, Adonitol, Maltotriose, 2-Deoxy Adenosine, Adenosine, Glycyl-L-Aspartic Acid, m-Inositol, D-Threonine, Fumaric Acid, Bromo Succinic Acid, Propionic Acid, Mucic Acid, Glycolic Acid, Glyoxylic Acid, D-Cellobiose, Inosine, Tricarballylic Acid, L-Serine, L-Threonine, L-Alanine, L-Alanyl-Glycine, Acetoacetic Acid, N-Acetyl-\(\beta\)-D-Mannosamine, Mono Methyl Succinate, Methyl Pyruvate, D-Malic Acid, L-Malic Acid, Glycyl-L-Proline, p-Hydroxy Phenyl Acetic Acid, m-Hydroxy Phenyl Acetic Acid, Tyramine, D-Psicose, Glucuronamide, Phenylethyl-amine, or 2-Aminoethane.