David Coil edited Results and Discussion.md  over 9 years ago

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Growth was only observed under aerobic conditions, from 4C to 28C, with optimal growth around 25C. Low levels of growth were observed at pH 5.5 and 7.9, maximum growth occured around neutral pH. Salt was not required for growth, and the strain could tolerate up to 5% NaCl, with optimal growth at 1% NaCl.  Strain Coronado(T) could use the following as sole carbon sources: Gly-Glu, L-Rhamnose, D-Mannose, D-Trehalose, a-D-Glucose, L-Fucose, D-Galactose, Citric acid, D-Glucuronic acid, D-Galactonic acid, L-Galactonic acid-g-Lactone, Acetoacetic acid, Acetic acid, Pyruvic acid, and L-Malic acid. The strain was unable to grow on N-Acetyl-D-Glucosamin, D-Saccharic Acid, Succinic Acid, L-Aspartic Acid, L-Proline, D-Alanine, Dulcitol, D-Serine, D-Sorbitol, Glycerol, D-Gluconic Acid, D,L-ALPHA-Glycerol-Phosphate, L-Lactic Acid, Formic Acid, D-Mannitol, L-Glutamic Acid,  **Phylogeny and Genome analysis**  Phylogenetic analysis was performed using the full length (1491bp) 16S rDNA sequence from the genome assembly, not the shorter (1350bp) version from Sanger sequencing. The Coronado(T) 16S rDNA sequence showed only 95% identity to the phylogenetically closest relative, _Porphyrobacter sanquineus_, and identity was even lower throughout the rest of the tree. Given the low 16S rDNA identity to other members of the family, we did not perform DNA-DNA hybridization as this would have been uninformative (\cite{STACKEBRANDT_1994}, \cite{Tindall_2010}