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David Coil edited Methods.md
over 9 years ago
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CATALASE AND OXIDASE?
Carbon source utilization was assayed using the Phenotypic MicroArray(TM) services offered by Biolog
Inc. METHODS STUFF HERE. Plate PM1 Inc following standard proceedures for gram-negative bacteria. Colonies were grown on blood agar at room temperature and suspended in IF-0a inoculating fluid (Biolog) to a density of 42% transmittance. The cell suspension was
performed diluted 1:6 in
triplicate, IF-0a plus 1x Dye H (Biolog) and
a carbon source utilization
MicroPlate (PM1; Biolog) was inoculated with 100μl per well. The PM1 microplate was
only scored if growth incubated at 23 degrees C and read by the OmniLog instrument every 15 minutes for 96 hours. Duplicate sets of OmniLog data were converted to average read value and a threshold of 78 was
observed required in
all three plates. both replicates for a positive call.
**Respiratory quinones, polar lips, and fatty acids**