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David Coil edited Body of paper.md
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**INTRODUCTION**
In this study, strain Coronado
T was isolated from a stadium seat at Niedermeyer Field, Coronado High School in Coronado, California, USA as part of a nationwide Citizen Science project (Project MERCCURI - www.spacemicrobes.org.) One goal of Project MERCCURI was to collect bacterial isolates to be used for an experiment aboard the International Space Station (ISS).
A cotton swab was used to swab the surface of a stadium seat, then colonies were isolated via plating onto agar plates. The 16S rRNA gene
was sequenced from this
particular isolate
and was at least 99% identical to rRNA genes from a few uncultured organisms. Uncultured isolates with high identity (=>99%) were found in samples from deep ocean sediment and the human skin microbiome. However, the highest identity to a cultured organism (_Porphyrobacter donghaensis_) \cite{15545463} was only 95.5% (as determined by BLAST, \cite{2231712}). Given the low identity to characterized species, a more detailed study of this isolate was undertaken.
A 2005 phylogenetic analysis of the _Alphaproteobacteria_ class led to the creation of a new family, _Erythrobacteraceae_, to house the genera _Erythrobacter_, _Porphyrobacter_ and _Erythromicrobium_ \cite{16166687}. These genera were later joined by _Altererythrobacter_ \cite{17911284} and _Croceicoccus_ \cite{19620383}, the latter work also emended the description of the family. Members of the _Erythrobacteraceae_ family are Gram-negative, aerobic bacteria that contain carotenoids, usually appearing pink, orange or yellow. They do not form spores, are chemo-organotrophic, and are most often associated with aquatic environments.