Jonathan A. Eisen edited Results and Discussion.md  over 8 years ago

Commit id: a98c37735ad9613b80a9bb11c40a3ded830b5266

deletions | additions      

       

Cells were non-motile and not observed to form spores or possess flagella, though over 30 flagella or flagella-associated genes are present in the genome. In contrast, the genome for a non-motile close relative (_Porphyrobacter cryptus_) does not contain any flagellar genes so it is possible that strain CoronadoT is motile under specific conditions. Unlike most members of the _Erythrobacteraceae family_, strain Coronado(T) is oxidase-negative. This strain is catalase-positive, and unable to hydrolze casein or starch.  Cells were oval or rod shaped and ranged in length from 1.2μm 1.2 μm  to 2.2μm 2.2 μm  with an average of 1.6μm 1.6 μm  (Figure 1). Cell width ranged from 0.6μm 0.6 μm  to 1μm 1.0 μm  with an average of 0.8μm. 0.8 μm.  Growth was only observed under aerobic conditions, from 4°C 4 °C  to 28°C, 28 °C,  with optimal growth around 25°C. 25 °C.  No growth was observed under microaerophilic conditions (culture caps closed). Low levels of growth were observed at pH 6.0 up to pH 8.0, maximum growth occurred around neutral pH. NaCl was required for growth, and the strain could not grow at >1.5% NaCl, optimal growth was at 0.5% NaCl. No statistically significant difference in growth was observed between earth and microgravity aboard the International Space Station (ISS). Strain CoronadoT could utilize the following as sole carbon sources: Glycyl-L-Glutamic Acid, L-Rhamnose, D-Mannose, D-Trehalose, a-D-Glucose, L-Fucose, D-Galactose, Citric acid, D-Glucuronic acid, D-Galactonic acid, L-Galactonic acid-\(\gamma\)-Lactone, Acetoacetic acid, Acetic acid, Pyruvic acid, and L-Malic acid.