David Coil edited Body of paper.md  over 9 years ago

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## Body of paper  A 2005 phylogentic analysis of the _Alphaproteobacteria_ class led to the creation of a new family, _Erythrobacteraceae_, to house the genera _Erythrobacter_, _Porphyrobacter_ and _Erythromicrobium_ \cite{Lee_2005}. \cite{Lee\_2005}.  These genera were latter joined by Altererythrobacter\cite{Kwon\_2007} _Altererythrobacter_\cite{Kwon\_2007}  and _Croceicoccus_\cite{Xu\_2009}, the latter work also ammending the description of the family. The salient features of the _Erythrobacteraceae_ family are Gram-negative, rod-shaped or pleomorphic coccoid bacteria that contain cartenoids. They do not form spores and are Chemo-organotrophic. Most species contain bacteriochlorophyll _a_. In this study, strain Coronado(T) was isolated from a stadium seat at Niedermeyer Field, Coronado High School in Coronado, California, USA as part of a nationwide Citizen Science project (Project MERCCURI) looking for isolates to send to the International Space Station (ISS) for a growth experiment. A cotton swab was used to swab the surface of a stadium seat, then dilution plated onto lysogeny-broth (LB) agar plates. Almost full length (primers 27F and 1391R) 16s rRNA sequencing showed 99% identity to a few uncultured sequences at NCBI, but only 95% identity to any cultured organsim (_Porphyrobacter donghaensis_)\cite{Yoon_2004}. Uncultured isolates with high identity (=>99%) were found in studies of deep ocean sediment and the human skin microbiome.