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##Sanger #Sanger  Sequence Processing The end product of Sanger sequencing is the production of sequences (reads) for each sample submitted. Upon receiving Sanger reads from a sequencing facility, typically via e-mail, it is necessary to do some pre-processing before they can be analyzed. These steps include quality trimming the reads, reverse complementing the reverse sequence, aligning the reads, generating a consensus sequence, and converting to FASTA format. Note - there are dozens of different formats used for sequence information. FASTA format is one of the simplest. In the FASTA format a sequence file is given a name in one line (the name follows the character '>') and then the sequence information is in the following lines. There are very limited options for free software that allow the user to perform these steps.