deletions | additions       diff --git a/Organism Identification using 16s rRNA gene sequence.md b/Organism Identification using 16s rRNA gene sequence.md  index 9189e45..62892b6 100644  --- a/Organism Identification using 16s rRNA gene sequence.md  +++ b/Organism Identification using 16s rRNA gene sequence.md 
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Paste in the two 16S rDNA sequences and click on the "BLAST" button. Unless both your sequence and the sequence to which you are comparing were amplified with the same primers, the query coverage will not be 100% (Figure 9). A low identity can be the result of poor sequence quality or taxonomic distance.   ##Choosing an organism to sequence  JENNA WILL EXPAND THIS SECTION  A choice of whether to sequence an organism based on these results depends on the project goal. For example, an identity of 100% suggests that at least at the 16S level, the candidate organism is very similar to what is already in the database. However, many organisms vary greatly in gene content between strains and an additional genome may still be informative. The use of 16S rRNA gene sequence percent identity as a proxy for species delimitation in bacteria is a subject of some debate in the field. \cite{Chan_2012}\cite{Drancourt_2005}\cite{Hanage_2006}\cite{Stackebrandt_2002}.