deletions | additions       diff --git a/Building a 16S rDNA Phylogenetic Tree.md b/Building a 16S rDNA Phylogenetic Tree.md  index 1f368d5..c775397 100644  --- a/Building a 16S rDNA Phylogenetic Tree.md  +++ b/Building a 16S rDNA Phylogenetic Tree.md 
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Our preferred approach to classifying microbial species is to place the unknown organism in the context of a phylogenetic tree using its 16S rRNA gene sequence. Building a phylogenetic tree from a 16S rRNA sequence is fairly straightforward, but the interpretation of the tree can be a bit complex. Here, we attempt to guide you through both. However, some complicated cases will require consultation with an expert in the field of phylogenetics or systematics.  The outline of the workflow is to use the Ribosomal Database Project (RDP) to generate an alignment of the sequence with close relatives and an outgroup. This is followed by cleanup of the RDP headers, tree-building with FastTree, FastTree \cite{19377059},  and viewing/interpretation of the tree using Dendroscope. Dendroscope \cite{22780991}.  ##Obtain an RDP alignment