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Jonathan A. Eisen edited Discussion.md
almost 10 years ago
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All of the scripts and programs for this workflow are open-source and available online for free to ensure that individual researchers and small groups are able to access and utilize the tools necessary to complete the workflow. In general, many available bioinformatic tools are free and open source, but the installation, operation and maintenance of these programs is often complex, requiring specific technical expertise or extensive detailed instructions and best practices in the field remain undefined.
It should also Sequencing, sharing, and publishing a genome sequence can certainly be
noted considered as an important process in its own right. Once a genome is shared other people can use that
genome for various and diverse purposes. However, just because one can stop after publishing and releasing a
sequenced genome
sequence that does not mean one should ignore what one can do with the data. A genome sequence is
simply also a starting
place point for
analysis. Analysis of the genome in detail, many computational and laboratory analyses that can provide insight into evolution, ecology, physiology, biochemistry, metabolism, and
comparative genomics more. Such analyses are
both outside beyond the scope of this
workflow. These additional steps could include analyses such as aligning the genome to a close relative to look for rearrangements (e.g. Progressive Mauve REF), phylogenomic analysis (e.g. what?), looking for evidence of horizontal gene transfer (e.g. how?), looking for novel functions within the lineage (how?), examining genomic context (e.g. JContextExplorer, REF) workflow and
phylogenomic profiling (REF). MORE STUFF HERE. paper but that should not be taken as implying they are not interesting, useful or important.