The ROTI index based on the variation of the TEC is used to detect and characterize the ionospheric irregularities. In the present work, we present a comparative study of five different methodologies to ROTI calculation in order to evaluate the most suitable for the Brazilian region. This was performed over three GNSS stations at different latitudes: São Luís (SALU, 2°31′ S, 44°16′ W; dip: -6.60°) that is located near the dip equator; Cachoeira Paulista (CHPI, 22°40’ S, 44°59’ W; dip: -35.99°) which set close to the southern crest of the EIA at low latitude); and Santa Maria (SMAR, 29° 41′ S, 53° 48′ W, dip: -43.51°) a low-to-mid latitude station close to center of the SAMA region. The period of analysis covered January and December 2015. Our results show that only one out of the five techniques proposed seems to be appropriated for ROTI construction in the Brazilian sector. Our results are supported by comparison of the ROTI with TEC maps obtained over Brazil, ionograms acquired at Fortaleza (FZA0M), SALU, and CHPI ionosonde stations, and All-Sky imagers collected at the São João do Cariri, and CHPI. In addition, we were able to observe the typical irregularities of the Brazilian ionosphere by using the ROTI which we have classified as EPB.