Nathan edited section_RESULTS_subsection_Environmental_isolates__.tex  over 8 years ago

Commit id: acbb723e91c9c54f195b6f91953bdd95595cffd9

deletions | additions      

       

\section{RESULTS}  \subsection{Environmental isolates are pathogenic toward experimental invertebrate  hosts} We analyzed the virulence of 18 \textit{S. maltophilia} isolates in two different experimental host systems. The first assay examines the ability of \textit{S. maltophilia} to resist predation by the bacterivorous amoeba \textit{Dictyostelium discoideum}. Bacterial cells were spread over agar plates and allowed to dry. Then, uniform aliquots of serially diluted \textit{D. discoideum} cells were spotted on top of the bacteria. The plates were incubated at room temperature in the dark and each dilution was scored for the presence or absence of a plaque, which indicated successful predation. Eight aliquots from each dilution were analyzed and non-linear regression was used to estimate the median plaque-forming dose (PD_{50}) for each isolate. As seen in \textbf{Table 2}, the apparent PD_{50} varied widely for the 18 \textit{S. maltophilia} isolates tested. Importantly, several environmental isolates (list) exhibited significant virulence toward amoeba.  The growth promotion potential of S. maltophilia isolates on canola seedlings under normal growing condition. In order to determine the potential of S. maltophilia isolates to promote growth in canola, 4 day old canola seedlings were inoculated overnight with bacterial cultures suspended in sterile distilled water. Percentage changes of the median in parameters measured were determined using the no bacterial control median as the initial and the isolate medians as the final under both stress and non-stress conditions.   No significant differences were observed in number of root branches and root length in canola plants exposed to bacterial isolates relative to the “no bacterial” control (Figs. 1 & 2). Stem length of canola plants, however, was significantly (P < 0.05) lower in canola seedlings inoculated with isolate CDC 92-03-30, ATCC 17666, CDC 98-43-10, CDC 2013-11-01 and CDC 2011-09-42 than the “no bacterial” isolate (Fig. 3).