deletions | additions       diff --git a/subsection_Effect_of_temperature_and__.tex b/subsection_Effect_of_temperature_and__.tex  index 02bed25..56c621e 100644  --- a/subsection_Effect_of_temperature_and__.tex  +++ b/subsection_Effect_of_temperature_and__.tex 
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Since immune function can vary throughout the lifecycle of insects (refs), we next sought to determine if the developmental stage of the host animal impacted susceptibility to \textit{F. tularensis} LVS. Serial dilutions of \textit{F. tularensis} cells were injected into the hemocoel of small juvenile (less than 1.5 cm total length), large juvenile (greater than 1.5 cm total length), adult female, and adult male OS cockroaches and survival was monitored for at least 7 days at 37°C. The LD_{50} values for small and large juveniles and adult females were very similar. Interestingly, adult male OS cockroaches were found to be slightly more susceptible to \textit{F. tularensis} infection (Table 1).  \subsection{Mammalian virulence factors are required for \textit{F. tularensis} virulence toward OS cockroaches}