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\subsection{Effect of temperatureand insect development  on \textit{F. tularensis} virulence} Transcriptional activation of \textit{F. tularensis} virulence factors is at least partially controlled by temperature \cite{18842136}. Since insects are ectothermic, taking on the ambient temperature of their surroundings, we sought to determine the impact of varied temperatures on \textit{F. tularensis} pathogenesis against the OS cockroach. Serial dilutions of \textit{F. tularensis} cells were injected into the hemocoel of juvenile OS cockroaches and their survival was monitored for at least 7 days at 40°C, 37°C, 30°C, and 22°C. We found that decreasing temperatures correlated with improved survival (Figure 3). The 40°C LD_{50} of \textit{F. tularensis} LVS was calculated to be 1.24 x 10^{4} CFU, while the 22°C group had an LD_{50} value of 2.0 x 10^{6} CFU. 3, Table 1).