Jason Huntley edited section_Methods_subsection_Bacterial_Strains__.tex  over 8 years ago

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\section{Methods}  \subsection{Bacterial Strains and Knockout Construction }  In this study, we used \textit{F. tularensis} LVS stocks provided by two different laboratories. The strain we denote designated  as \textit{F. tularensis} LVS-1 is was provided by  the J.F.  Huntley lab stock strain and laboratory at  the parental isolate University  of Toledo (from ATCC). LVS-1 was  the parental strain use to generate both  \textit{ΔdsbA} and \textit{ΔdipA} mutants. The strain we denote designated  as \textit{F. tularensis} LVS-2 is was provided by  the J.  Horzempa lab stock strain and laboratory at West Liberty University (from ????). LVS-2 was  the parental isolate of the strain used to generate both  \textit{ΔdeoB} and \textit{ΔiglC} mutants.There was no discernible difference in virulence between the two parental isolates (Table 1).  \textbf{Jason, can you add a paragraph describing the construction of the \textit{ΔdsbA} and \textit{ΔdipA} mutants?}