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\textbf{Table 1.} The median lethal dose, LD_{50}, of \textit{F. tularensis} LVS parental strains, LVS-1 or LVS-2, and deletion mutants thereof lacking known virulence factors. The two different LVS strains exhibited no significant difference in LD_{50}. LD_50.  All four mutants, previously shown to be attenuated in other models, also were significantly attenuated in the cockroach with increased LD_50 values. Lethal doses 50\% LD_50  and corresponding 95\% confidence intervals were estimated by non-linear regression using the drc package in the R programming environment \cite{Ritz}.