Nathan edited Unfortunately_suppliers_of_wax_worms__.tex  over 8 years ago

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Unfortunately, suppliers of wax worms and OS cockroaches are generally focused on non-scientific audiences. Wax worms are a popular choice for fishing bait and both wax worms and OS cockroaches are used as food for captive reptiles. In these markets, easy handling by consumers is critically important. This has led commercial suppliers to inactivate the wax worm silk gland by some unknown procedure (possibly using a freeze treatment as described by hobbyists in online forums \cite{OpenBugFarm,BestBetWormKits}). ).  The physiological and immunological impacts of silk gland dysfunction are unknown, but it is clear that this organ is an import part of the antibacterial response in \textit{G. mellonella} \cite{19414015}. To avoid this serious complication for pathogenesis studies, wax worms can be reared in the laboratory \cite{23271509} but we found it difficult to consistently do so without microbial contamination, a factor that might contribute to unpredictable rates of background mortality in our and others' studies \cite{2340270,26388863,26379240,18195031}}. In contrast, maintenance of a cockroach breeding colony in the laboratory is simple and straightforward \cite{7966174,14272467}. Compared to other cockroach species, OS cockroaches are docile and easy to handle. They are relatively slow, remain immobile when placed on their back, do not climb vertical glass or plastic surfaces, and they do not fly. It is unclear if these are characteristics of wild OS cockroaches or if they have been selected during captive breeding. Importantly, a minimal amount of maintenance is required to prevent microbial contamination (and odor) in OS cockroach breeding colonies. As a result, we rarely observe mortality in uninfected control groups of OS cockroaches (\textbf{Figure 1} and \textbf{Table 2}).