Jason Huntley edited section_Results_subsection_Challenge_of__.tex  over 8 years ago

Commit id: b810e93477669ae6c3ac1a2b77536eed0be89aa1

deletions | additions      

       

\section{Results}  \subsection{Challenge of \textit{B. dubia} with \textit{F. tularensis} LVS}  To determine if OS cockroach survival was proportional to the number of bacteria present in the challenge inoculum, serial dilutions of \textit{F. tularensis} LVS were injected into into the hemocoel of juvenile cockroaches that measured greater than 1.5 cm in length. Challenged cockroaches were housed at 37$^{\circ}$C and survival was monitored for 7 days. The percentage of each group that survived challenge and the rate of death were dose-dependent; all no  OS cockroaches challenged survived challenge  with 10^{5} CFU of \textit{F. tularensis} LVS and all cockroaches  died by day  five days post-challenge while only a single cockroach post-challenge; 60% of cockroaches  challenged with 10^{3} 10^{4}  CFU of \textit{F. tularensis} LVS died during the course survived through day seven post-challenge; and 90%  of the experiment cockroaches challenged with 10^{3} CFU of \textit{F. tularensis} LVS survived through day seven post-challenge  (Figure 2). Injection of PBS alone did not result in any deaths.