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Nathan added Like_fruit_flies_and_wax__.tex
over 8 years ago
Commit id: 9718fbab2700fc5860fd0c7d6cd9d5172d11b4e0
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Like fruit flies and wax moth larvae, OS cockroaches are readily available. Several strain repositories supply the scientific community with \textit{D. melanogaster} seed stocks and in-house rearing is easily accomplished using well-established protocols \cite{21709308, 17921418}. Unfortunately, \textit{D. melanogaster} does not tolerate incubation at mammalian body temperatures and quantitative infection requires highly specialized equipment. Thus, we did not consider \textit{D. melanogaster} for our studies. In contrast, both \textit{G. mellonella} and \texit{B. dubia} survive at mammalian body temperatures. Critically, both of these hosts are able to mount effective immune responses against non-pathogenic microorganisms while infection by \textit{F. tularensis} results in dose-dependent mortality (\cite{17400503}; \textbf{Figure 1} and \textbf{Table 1}). Thus, \textit{F. tularensis} LVS is able to evade active immune functions and establish a lethal infection in both of these experimental hosts.
