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\subsection{Effect of \textit{B. dubia} development on \textit{F. tularensis} virulence}  Because insect immune responses are known to vary by age and developmental stage \cite{25730277}(other refs), we sought out to determine if there were any differences in susceptibility to infection in late instar juvenile cockroaches(as was used  in all other portions of this report) compared to comparison with  adult male and adult female cockroaches. We also  attempted toalso  evaluate susceptibility in early instar cockroaches, cockroaches (size < 1 cm total length),  but these groups  had significant number large numbers  of animals die that died  due to trauma and were, therefore, excluded from subsequent analysis. Injections in adults were performed using the same method described for the juveniles. We found that adult female and juvenile cockroaches had susceptibility patterns that were statistically indestinguishable from each other. But interestingly, we saw that adult male cockroaches were more sensitive to killing by \textit{F. tularensis}. tularensis} (Figure 3, Table 1).