deletions | additions       diff --git a/section_Methods_Bacterial_Strains_and__1.tex b/section_Methods_Bacterial_Strains_and__1.tex  index ded6e62..7adb04c 100644  --- a/section_Methods_Bacterial_Strains_and__1.tex  +++ b/section_Methods_Bacterial_Strains_and__1.tex 
...
\section{Methods}  Bacterial Strains and Knockout Construction \textbf{Need Joe and Jason (and others in their labs, if applicable) to edit this section.}  The wild type Francisella tularensis LVS strain, along with the ΔdsbA and ΔdipA mutants, were provided by Jason Huntley (University of Toledo). The ΔiglC and ΔdeoB mutants were provided by Joseph Horzempa (West Liberty University). Deletion mutants were constructed using the pJH1/pGUTS system. A deletion plasmid was created using the pJH1 backbone and mobilized into F. tularensis LVS via conjugation. Exoconjugates were transformed with pGUTS using electroporation and screened for deletion of target gene. Lastly, pGUTS was cured from the deletion mutant to create the completed deletion mutants.  Bacterial Growth Conditions 
...