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\subsection{Effect of \textit{B. dubia} development on \textit{F. tularensis} virulence}  Because insect immune responses are known to vary by age and developmental stage \cite{25730277}(other refs), we sought to determine if there were any differences in susceptibility to infection in late instar juvenile cockroaches in comparison with adult male and adult female cockroaches. We also attempted to evaluate susceptibility in early instar cockroaches (size < 1 cm total length), but these groups had large numbers of animals that died due to injection  trauma and were, therefore, excluded from subsequent analysis. Injections in adults were performed using the same method described for the juveniles. We found that although the overall percentages of surviving cockroaches differed between adult female (50 percent) and juvenile (30 percent) cockroaches, both groups were equally susceptible to \textit{F. tularensis} LVS infections (no statistical difference between groups). By comparison, adult male cockroaches were more sensitive to killing by \textit{F. tularensis}, with no male cockroaches alive after day 6 post-infection (Figure 4, Table 1).