Jason Huntley edited We_previously_determined_that_the__.tex  over 8 years ago

Commit id: 803a6a02bf2b76df1b60346099f48ec8941637ec

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We previously determined that the Madagascar hissing (MH) cockroach (\textit{Gromphadorhina laevigata}) met these initial the above noted  criteria and is was  a suitable surrogate host for the facultatively facultative  intracellular pathogens \textit{Burkholderia mallei}, \textit{B. pseudomallei}, and \textit{B. thailandensis} \cite{22892068}. Here, we have extended sought to extend  the use of tropical cockroach species as experimental hosts for other  bacterial pathogenesis studies. studies, including \textit{F. tularensis}.  The Orange Spotted (OS) cockroach (Figure 1, \textit{Blaptica dubia} Serville 1839) also meets the above criteria for as  an ideal insect host for pathogenesis studies but is more readily available from commercial suppliers and is more docile compared to the MH cockroach. The OS cockroach does not vocalize like the MH cockroach and itwill  usually will  remain in an immobile defensive position when placed on its back. Of interest to researchers who desire to rear their own animals, host species,  the OS cockroach does not climb vertical glass or plastic surfaces, which aids in constraining containment of  the animals to cockroaches in  their rearing containers. In this study, we found the OS cockroach to be a permissive host for \textit{F. tularensis} LVS and the non-pathogenic near-neighbor \textit{Francisella philomiragia} \cite{22628499}. Lethality depended upon the dose of bacteria given, the temperature of incubation, and, interestingly, the developmental stage and gender of the host animal. cockroach.  Intracellular and extracellular bacterial titers increased throughout the course of infection and several mutant strains lacking factors known to be involved in \textit{Francisella} pathogenesis were attenuated. These results demonstrate that the OS cockroach is an available a viable  alternative to mammals and other insect species for the study of \textit{F. tularensis} and possibly other pathogenic bacteria. bacteria which have a vector-borne stage.