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\subsection{\textit{In vivo} growth of \textit{F. tularensis} LVS}    An in vivo In order to determine the kinetics of \textit{F. tularensis} LVS  growth curve during infection, we harvested hemolymph from infected OS cockroaches at various time points post inoculation. The antibiotic gentamicin  was characterized within B. dubia roaches where both used to distinguish between  intracellular (resistant)  and extracellular growth was observed. total (sensitive) bacterial loads.  After inoculation of 106 10^{6} CFU, \textit{F. tularensis} LVS quickly multiplied, reaching 10^{9}  CFU per ml  of F. tularensis LVS, total growth to 109 cells was observed after hemolymph by  96hours. Two  hours after treatment with gentamicin revealed a small fraction of (Figure 2). Interestingly, most bacteria  in vivo growth was intracellular. After 96 hours post initial inoculation of B. dubia roaches with F. tularensis LVS, 108 cells the hemolymph  were classified as intracellular. A steady increase in CFU is observed in both total and intracellular growth, as show in figure 2. gentamicin sensitive, indicating that they were located outside of host cells.