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\subsection{\textit{In vivo} growth of \textit{F. tularensis} LVS}    In order to monitor the kinetics of \textit{F. tularensis} LVS growth during infection, we harvested hemolymph from infected OS cockroaches at various time points post-infection. The antibiotic gentamicin was used to distinguish between intracellular and total bacterial loads. Since gentamicin does not penetrate eukaryotic host cells, it is active only toward bacterial that are located extracellularly during the exposure period \cite{20865166, 21628658}.  After an initial inoculum of 10^{6} CFU, \textit{F. tularensis} LVS quickly replicated, reaching 10^{9} CFU per ml of hemolymph by 96 hours (Figure 5). (\textbf{Figure 5}).  Interestingly, most bacteria in the hemolymph were sensitive to gentamicin, indicating that they were located outside of host cells exposed to the extracellular environment  at some time during the 2 hours prior to harvest.Similar proportions of extracellular to intracellular \textit{Francisella} cells have been reported in fruit flies \cite{20865166}.