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Jason Huntley edited subsection_Effect_of_textit_B__.tex
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\subsection{Effect of \textit{B. dubia} development on \textit{F. tularensis} virulence}
Because insect immune responses are known to vary by age and developmental stage \cite{25730277}(other refs), we sought to determine if there were any differences in susceptibility to infection in late instar juvenile cockroaches in comparison with adult male and adult female cockroaches. We also attempted to evaluate susceptibility in early instar cockroaches (size < 1 cm total length), but these groups had large numbers of animals that died due to trauma and were, therefore, excluded from subsequent analysis. Injections in adults were performed using the same method described for the juveniles. We found that
although the overall percentages of surviving cockroaches differed between adult female
(50 percent) and juvenile
cockroaches (30 percent) cockroaches, both groups were equally susceptible to \textit{F. tularensis} LVS infections (no
statistically statistical difference between groups).
Interestingly, as compared with adult female or juvenile cockroaches, By comparison, adult male cockroaches were more sensitive to killing by \textit{F.
tularensis} tularensis}, with all male cockroaches dead by day 5 post-infection (Figure 4, Table 1).