deletions | additions       diff --git a/subsection_Effect_of_temperature_and__.tex b/subsection_Effect_of_temperature_and__.tex  index 56c621e..d919100 100644  --- a/subsection_Effect_of_temperature_and__.tex  +++ b/subsection_Effect_of_temperature_and__.tex 
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\subsection{Effect of temperature and insect development on \textit{F. tularensis} virulence}  Transcriptional activation of \textit{F. tularensis} virulence factors is at least particially partially  controlled by temperature (ref). Since insects are ectothermic, taking on the ambient temperature of their surroundings, we sought to determine the impact of varied temperatures on \textit{F. tularensis} pathogenesis against the OS cockroach. Serial dilutions of \textit{F. tularensis} cells were injected into the hemocoel of juvenile OS cockroaches and their survival was monitored for at least 7 days at 40°C, 37°C, 30°C, and 22°C. A decrease in temperature We found that decreasing temperatures  correlated to a increase in with increasing  LD_{50} value values  and median time times  to death (Table 1). The 40°C LD_{50} of \textit{F. tularensis} LVS was calculated to be 1.24 x 10^{4} CFU, while the 22°C group had an LD_{50} value of 2.0 x 10^{6} CFU.Since immune function can vary throughout the lifecycle of insects (refs), we next sought to determine if the developmental stage of the host animal impacted susceptibility to \textit{F. tularensis} LVS. Serial dilutions of \textit{F. tularensis} cells were injected into the hemocoel of small juvenile (less than 1.5 cm total length), large juvenile (greater than 1.5 cm total length), adult female, and adult male OS cockroaches and survival was monitored for at least 7 days at 37°C. The LD_{50} values for small and large juveniles and adult females were very similar. Interestingly, adult male OS cockroaches were found to be slightly more susceptible to \textit{F. tularensis} infection (Table 1).