deletions | additions       diff --git a/Both_wax_worms_and_cockroaches__.tex b/Both_wax_worms_and_cockroaches__.tex  index c8abdea..cc347b9 100644  --- a/Both_wax_worms_and_cockroaches__.tex  +++ b/Both_wax_worms_and_cockroaches__.tex 
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Both wax worms and cockroaches can be infected with known doses of microorganisms using needle and syringe combinations \cite{23271509, 22892068}. But unlike wax worms, OS cockroaches can also be infected using sharpened gel-loading pipette tips, which increases the safety and decreases the cost associated with pathogenesis studies in this host. After infection, wax worms can survive at least one subsequent administration of antibiotics \cite{17400503, 23402703}. Here, we established that OS cockroaches can tolerate at least 3 injections following infection without an increase in background mortality (\textbf{Table 2}). Importantly, the experimental window available to investigators is substantially different between wax worms and OS cockroaches. OS cockroaches undergo incomplete metamorphosis, with each developmental stage (or instar) lasting between 20 and 45 days. In total, it takes approximately 6 months for OS cockroaches to reach adulthood. The juvenile cockroaches used in this study were infected during the 6th instar (next to last), leaving between 30 and 60 days of experimental observation before they would have molted into the adulthood. In contrast, we often observed that considerable fractions (>25\% in some cases, data not shown) of wax moth larvae pupated during a typical 7 day survival experiment. This is troublesome for studies of host-pathogen interactions because wax worm immune responses are known to vary throughout the period leading up to pupation \cite{17198709}. Thus, small differences in individual age may impact the immune status of wax worms. While we did observe that adult male cockroaches were more susceptible to \textit{F. tularensis} (\textbf{Figure 5}), the similarity between mortality in juvenile and adult female cockroaches indicates that small differences in age are unlikely to effect experimental results in this system. Collectively, these differences demonstrate that OS cockroaches offer important improvements compared to wax worms for studies of microbial pathogenesis. Thus, we went on to characterize several relevant factors in this model, including temperature, the impact of temperature on virulence,  intracellular versus extracellular growth, and the usefulness of the model for pharmacological screening.