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\subsection{Effect of temperature on \textit{F. tularensis} virulence}  Transcriptional activation of \textit{F. tularensis} virulence factors is at least partially controlled by temperature \cite{18842136}. Since insects are ectothermic, taking on the ambient temperature of their surroundings, we sought to determine the impact of varied temperatures on \textit{F. tularensis} pathogenesis inside the OS cockroach. Serial dilutions of \textit{F. tularensis} were injected into the hemocoel of juvenile OS cockroaches and their survival was monitored for at least 7 days at 22°C, 30°C, 37°C, or 40°C. Overall, we found that higher temperatures correlated with higher LVS virulence in the OS cockroach  (Figure 3, Table 1). No OS cockroaches survived challenge infection  with \textit{F. tularensis} LVS at 37°C or 40°C and all cockroaches at those these  two higher temperatures were dead by day five post-infection. By comparison, 20 percent of cockroaches survived \textit{F. tularensis} LVS infection at 30°C and 30 percent of cockroaches survived \textit{F. tularensis} LVS infection at 22°C.