David edited subsection_Pharyngeal_pumping_is_affected__.tex  about 8 years ago

Commit id: f8affa5742772ea8b8b3ac06baf4af0ad49e9918

deletions | additions      

       

\subsection{Pharyngeal pumping is affected by the concentration and the flow rate of bacterial food.}   seriously  consider adding the 10x data with the rates for four ODs from the previous version. > To demonstrate our method, we assayed pharyngeal pumping in the presence of steady state or oscillating food concentrations. Unless stated otherwise, the high and low food concentrations were set to OD$_{600} = 0.0$ and $4.0$, respectively.  We use our method to measure pumping behavior for animals at no food to high food levels (OD$_{600}$ 0.0 and 4.0).  The distribution of instantaneous pumping rates looks bimodal, with a peak at lower frequencies and a peak at higher frequencies Fig. (\ref{fig:results}A). The animals modulate their feeding behavior in the chips in response to the flow speed. When no food is present, the animals have a non-zero pumping rate as previously reported (Avery? Horvitz, Fig. \ref{fig:results}B). The mean rates for high food are comparable to the previously reported maximum pumping rates, between 3-4 Hz. Due to the bimodal nature of the pumping histogram, we define an on- and off-state. The on-state is defined as a continuous stretch of pumps that are 250 ms or less apart. The duty ratio is the fraction of time the animal spends in the on-state, which increases with the amount of food available.