deletions | additions       diff --git a/Here_we_describe_an_affordable__.tex b/Here_we_describe_an_affordable__.tex  index d836241..c26bebb 100644  --- a/Here_we_describe_an_affordable__.tex  +++ b/Here_we_describe_an_affordable__.tex 
...
Here we describe an affordable and scalable method for automatically assaying pharyngeal pumping. Our method combines our a  previously described microfluidic device \cite{kopito2014durable}, low cost educational microscopes, and an image analysis pipeline implemented using top tier open source tools and libraries, among the most widely used by researchers. The advantages of our approach include precise control of conditions such as the quality, uniformity, and concentration of available food, the possibility of prolonged measurement durations (hours, if required), unbiased automatic detection of pumping events, and the possibility to assay feeding conditions that change dynamically in a controlled manner. Manual scoring of pumping is arduous and limited to brief measurement periods. Automatic scoring of pumping on a high quality microscope is throughput-limited due to the cost of the imaging setup. equipment.  We found that lower quality imaging can be compensated for by rapid sampling and improved analysis without compromising the quality of the data. Using three microscopes we were able to assay up to 50 animals a per  day, i.e., 6 animals per objective, each for a full hour, at the approximate cost of $\$2,000$ (not including optional syringe pumps). The setup can be further duplicated and the rate limiting step is the  number of microfluidic devices that the researcher can load. The flexibility degree to which food availability can be  dynamically controlling the feeding conditions controlled  is dictated by the sophistication design of the microfluidic device used to restrict the motion of the animals and by the capabilities  of the pressure source that is driving the fluid flow.