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\subsection{Pharyngeal pumping is affected by the concentration and the flow rate of bacterial food.}   To demonstrate our method, we first assayed pharyngeal pumping in the presence of fixed food concentrations. In agreement with previous reports, pumping rates decreased when the concentration of bacteria was lowered but did not vanish in the absence of food (Fig. \ref{fig:results}A-C). In addition, the mean rates we observed were comparable (Fig. \ref{fig:results}missingA) to the values reported previously \cite{avery1993motor,hobson2006ser,findAveryPaper}. \cite{avery1993motor,hobson2006ser,avery1995}.  Henceforth, unless stated otherwise the high and low food concentrations were set to OD$_{600} = 0.0$ and $4.0$, respectively. Fig. \ref{fig:results}A-B depict typical distributions of instantaneous pumping rates under these conditions. When food was readily available, wild-type animals exhibited a peak rate of $4-5$ Hz but also a substantial tail of lower rates. In our hands, changing the flow rate in the device from $5$ to $200$ $\mu l / min$ resulted in a mild yet significant reduction in pumping rates (Fig. \ref{fig:results}A, C) and duty ratios (Fig. \ref{fig:results}D). Our measurements were performed continuously and therefore invariably included periods devoid of pumping events, as indicated by the low frequency tail in Fig. \ref{fig:results}A. Therefore, we also measured the duration of continuous pumping, defined as a series of consecutive pumps that were separated by no longer than $250$ ms. The fraction of time spent in continuous pumping was defined as the pharyngeal duty ratio, which also increased with food concentration (Fig. \ref{fig:results}D). These results indicate that our method provides an unbiased and efficient approach for characterizing pharyngeal pumping at precisely controlled steady state conditions.