deletions | additions       diff --git a/subsection_Pumping_rates_are_reduced__.tex b/subsection_Pumping_rates_are_reduced__.tex  index 46dc384..df6d18b 100644  --- a/subsection_Pumping_rates_are_reduced__.tex  +++ b/subsection_Pumping_rates_are_reduced__.tex 
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Combined, controlling food availability and prolonged measurements enable to assay pharyngeal pumping in a dynamic environment. To demonstrate this, we have oscillated the concentration of available food between its high ($D_{600}=4.0$) and low ($0.0$) values with a period of $360$ sec. Switching between the two food reservoirs was performed using a small volume rotative valve, which eliminates back-flow and cross contamination and minimizes flow disruptions. As a result, we could not detect a response to switching between two reservoirs containing the same concentration of food (data not shown).   Panels E-G in Fig. \ref{fig:results} depict examples of instantaneous pumping rates of individual animals and the corresponding food levels, as well as averaged data. Our analysis could readily detect changes in pumping rates that occurred over $\leq 10$ sec and in this assay the animals experienced high food availability for $90$ sec during each period. We thus expected that pumping rates would track the changes in food availability and that their highest and lowest mean pumping rates would be comparable to those measured at the corresponding fixed concentrations (\ref{fig:results}C). (Fig. \ref{fig:results}C).  Surprisingly, mean  pumping rates in the dynamic environment were $2-$ fold approximately $2-$fold  lower than the corresponding steady state rates (\ref{fig:results}F-G). ... observed mean (Fig. \ref{fig:results}F-G). Moreover, is was not uncommon for animals to maintain a low or high pumping rate regardless for up to $10$ min regardlesss of the oscillations in food concentration (see arrows in Fig. \ref{fig:results} and Fig. \ref{fig:results}I).