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_Amoeba Isolation/Culturing_: To enrich for amoeba, samples will be inoculated onto plates containing ???? media (1 mL 0.5 M MOPS buffer, 0.1 g yeast extract, 0.1 g tryptone, 15 g agar and 1X seawater base to make 1L) with a marine bacterium as a food source. Upon formation, plaques will be picked from the plate and inoculated into flasks containing approximately 10 mL of liquid media (no agar added). Flasks will be monitored for amoeba growth.  -algae + protist media recipes  http://www.ccap.ac.uk/pdfrecipes.htm  -amoeba isolation  http://www.bms.ed.ac.uk/research/others/smaciver/Protocols/AmoebaProts/isolation_of_amoebae.htm 
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After identification and imaging, isolates will be sent to ........ where they will be openly available.  - Where will we put the isolates (what culture collections?) - atcc? ccap (culture collection of algae and protezoa)  Addtionally, we will post the microscopy images, 18S sequences and phylogenies periodically to our website as blog posts (???).  All sequence data will be deposited in ..... **add this to above sections too**