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_Culturing/Isolation_: We will sample rhizospheric sediment as well as _Z. marina_ roots and leaves to use as isolation innoculum. We will initially focus on a targeted list of **taxa** (subset of clades where we expect to see members associating with seagrass based on a) literature and b) personal microscopy?) and subsequently expand our culturing to include representatives from all of the microbial eukaryotic lineages.   _Isolation/Culturing Rationale_: Microbial eukaryotes are extremely diverse, made up of multiple lineages with each containing numerous marine taxa (CITE). Because we do not know the true scope of this diversity yet in seagrass beds, we will initially focus isolation and culturing methods on targeted **taxa(??)** (taxanomic groups?) that we know associate with seagrass and rhizospheric sediment based on microscopy. Following the acquisition of initial sequence data and microscopy we will adjust our isolation and culturing efforts to include representatives from all microbial eukaryotic lineages. Our initial culturing efforts will focus on diatoms, algae, amoeba, and fungi **(??)**. We have seen representatives of each of these groups microscopically in _Z. marina_ samples, and we have had some success isolating amoeba from rhizosphere sediment. Additionally, our initial sequencing results using the ITS region suggest high levels of unidentified fungal diversity from _Z. marina_ leaves, roots and rhizosphere samples, so we have chosen to include them in our initial isolation and culturing efforts. **Say something about diatoms and algae? ** algae?**  Epiphitic algae can be visible on the outside of seagrass leaves and have been studied (?)...