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The V4 and V9 regions of the 18S rRNA gene will be amplified as in Aim 1 to identify isolated organisms and Sanger sequenced. Sequences with homology will be found using the SILVA database and Genbank searches. Phylogenies will then be built using RAxML and microeukaryotic taxonomies will be assigned based on tree position.  _Imaging_:  Isolates will be fixed and imaged under natural light. Fixed isolates will be stained for alpha tubulin in combination with staining using a general eukaryotic rRNA probe as in \cite{Hirst_2011}.  Multi species: same methods as Zmarina  Culture collections: Use Zostera from Bodega Bay (maybe try from different locations, different genotypes, etc.).