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**Aim 2: A global census of microbial eukaryotes found in association with leaves, roots, and sediment of the Order Alismatales, which includes three, independent lineages of seagrasses**  Aim 2 seeks to expand Aim 1 to include multiple species of seagrass as well as other aquatic plant species in the Order Alismatales. This expansion will allow for investigation into additional questions including: Are there distinct microeukaryotic communities associating with different host plants? Is there evidence of a phylogenetic signal or pattern between host plant species and their microeukaryotes? For locations with multiple host  species co-occurring, do we see the same or different associations? Are there any microeukaryotes that are shared between the three lineages of seagrass or unique to each lineage? **Aim 3: Establish culture collection of microbial eukaryotes associated with Zostera marina in Bodega Bay, California.**