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_Phenotypic and Molecular Characterization of Isolates_:  Microscopy of X-day old cultures will be used to record external morphology, and growth rate will be characterized at 10, 20, 30 deg C. Isolates will be fixed and imaged under natural light. Fixed Fungal isolates will be stained with fungal specific rRNA probes using flourescence in situ hybridization (FISH). All non-fungi  isolates will be stained for alpha tubulin in combination with FISH  staining using a general eukaryotic rRNA probe as in \cite{Hirst_2011}. If necessary, a more specific rRNA probe may also be used based on the group-specific sequence obtained.*I need to double check but I think fungi won't stain with alpha-tubulin (cilliates, protists, amoeba, will tho)  Molecular taxonomic classification of isolates will be performed using barcoding, first with V4 18S rDNA, then with group-specific markers, as outlined by the CBOL Protist Working Group \cite{Pawlowski_2012}. We will employ our published bioinformatic workflow \cite{Dunitz_2015} to infer phylogenetic placement of the isolates in the context of the most closely related type strains available.