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####**Aim 3: Establish culture collection of microbial eukaryotes associated with _Zostera marina_ in Bodega Bay, California.**  Microeuks diverse, multiple groups, marine in every lineage. We will focus on x, y, z because XXX. However, will adjust depending on sequence data. Detailed below is description on how we will culture a subset of these based on previous experience and what we have already had success culturing (Amoeba, fungi, ciliates). We plan to expand this initial culturing effort to include representatives of all groups (list groups) as dictated by sequence data.  _Culturing/Isolation_: Rhizospheric sediment as well as _Z. marina_ roots and leaves will be collected to use as isolation innoculum. A variety of culturing conditions will be used to target fungi, amoeba ...  A variety of media recipes found previously successful at isolating seagrass-associated fungi will be used here. These include potato dextrose agar, malt-dextrose agar, glucose peptone yeast agar and cornmeal agar, with all media being supplemented with salt and antibiotics \cite{Torta_2014}\cite{Vohn_k_2015}\cite{Panno_2013}. Media will also be made using Z. marina as the carbon source as was done by Torta el al \cite{Torta_2014} using the seagrass, Posidonia oceanica.