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###Rationale and Significance (3/4 page)  **briefly cite literature that will support, in greater detail, the eistence of the knowledge gap or need**  Although certain pathogenic microeukaryotes have been studied in great detail (ex. girardia), environmental microeukaryotes, specifically marine microeukatyores, are grossly uncharacterized. uncharacterized despite their important functional roles in their ecosystems.  \cite{Massana_2008} - "the relatively low number of microbial species actually described is largely because of the under-representation of microbial diversity in culture collections. The availability of powerful and relatively cheap sequencing techniques will be essential to determine the dimensions of such diversity. Another interesting point is that the increase in Novel  marine eukaryotic diversity occurs microeukaryotic lineages have previously been found  atalmost  allpossible  phylogenetic scales. Thus, putative high-rank groups occur, scales with sequencing \cite{Massana_2008}. However, many of these  novel clades within supergroups organisms are still a mystery to us as they  have been identified, yet to be cultured  and novel diversity it  is detected within all known lineages, from closely new species, genera, families, or orders. The challenge estimated that the total diversity of microbial eukaryotes  is to retrieve much higher (**how much higher?**) than what we currently have  in culture the organisms responsible for such sequences and to determine their trophic role and ecological function." \cite{Mora_2011}\cite{Pawlowski_2012}. For example, only around 550 marine fungi currently have cultured representatives when it is estimated that there are at at least 10,000 marine species \cite{Jones_2011}.  \cite{Sogin_2006} - the beginning of introduction Seagrass wasting disease, _Labyrinthula zosterae_,  is GREAT at explaining how important microeuks are a microeukaryote, that is desolating seagrass beds globally by causing dieback (REF). There is currently no genomic or transcriptomic data available for this organism  in the ocean NCBI.  Estimates indicate that known microeukaryotic diversity is significanly less than expected total diversity \cite{Mora_2011}  It is estimated that there are at least 10,000 species of marine fungi, but only around 550 currently have cultured representatives \cite{Jones_2011}.   Marine fungi represent a vast, untapped reservoir of bioactive secondary metabolites \cite{Imhoff_2016}.  \cite{Pawlowski_2012} - this has some #s on currently known diversity + estimates in a section on "The Unknown Vastness of Protist Richness"  Seagrass wasting disease (slime mold - Labyrinthula) is a microeukaryote and the disease is a huge problem for seagrass health/continues to desolate seagrass beds globally - there is currently no genome (or RNAseq data - prob. easier than doing genome b/c introns/exons) for Labyrinthula zosterae that I can find in NCBI/JGI Gold & looking at papers it doesn't seem hard to culture  **additional detail on why gap is a problem**    Fungal associations have been found to have beneficial effects on terrestrial plant fitness; for example, mycorrhizal fungi are involved in facilitating phosphorus and nitrogen uptake for their hosts. Microeukaryotes Marine fungi represent a vast, untapped reservoir of bioactive secondary metabolites \cite{Imhoff_2016}.  _Microeukaryotes  play a variety of important roles in coastal and marine ecosystems; they are primary producers, predators/prey (missing trophic level), decomposers, involved in biogeochemical cycling, untapped sources of natural products, potential pathogens and symbionts. symbionts._ **This is identical to what is said in Conceptual Framwork Section**  make sure to mention this somewhere:   -looked at microbial community in biofilm of Enhalus (both bacteria and eukaryotes--18s--) Their study suggests that seagrasses could be vectors for coral pathogens **describe why what you want to do is creative, original, and if credible, potentially transformative**  comparing We are proposing to use our  molecular sequence data as a benchmark by which to drive  and culture-based diversities, assess our culturing effort. This creative approach will ultimately result in a more successful culturing effort.  Microbial eukaryotes have never been investigated in this manner in _Z. marina_ beds.  unexplored ecosystem, first to use sequencing to characterize microeuks associated with _Z. marina_, we have 16S data + tons of metadata (ZEN) to enrich analysis **make explicit statement about what your contribution will be**